Molecular detection of cattle Sarcocystis spp. in North-West Italy highlights their association with bovine eosinophilic myositis
نویسندگان
چکیده
Abstract Background Cattle are intermediate hosts of six Sarcocystis species, among which hominis and heydorni can infect humans through the consumption raw or undercooked meat. In addition to zoonotic potential, there is increasing interest in these protozoa because evidence supporting role spp. occurrence bovine eosinophilic myositis (BEM), a specific inflammatory myopathy leads carcass condemnation considerable economic losses. Actually, all prevalence studies carried out on cattle Italy have been based either morphological 18S rDNA-based molecular techniques, most likely leading misidentification closely related species. Therefore, strong need for new data different their association with myositis. Methods To reach our aim, individual striated muscle samples from BEM condemned carcasses ( N = 54) diaphragm randomly sampled 59) were obtained Northwest slaughterhouses. Genomic DNA was extracted analyzed by multiplex-PCR targeting rDNA cox1 genes. PCR products amplified using genus-specific primer set absence fragment S. hirsuta , cruzi bovifelis sequenced achieve species identification. Results detected 67.8% slaughter 90.7% carcasses. identified as prevalent (61%), followed (10.2%), (8.5%) (1.7%). Notably, detected, presence significantly higher isolated (46.3% 40.7% respectively), while no statistically significant difference between (42.6% 1.8%, respectively) Furthermore, sequence analysis revealed putative two Conclusions Our study contributes updating Italy, highlighting three spp., lesions allowing us speculate possible major sarcosporidian involved Graphical
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ژورنال
عنوان ژورنال: Parasites & Vectors
سال: 2021
ISSN: ['1756-3305']
DOI: https://doi.org/10.1186/s13071-021-04722-5